Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro.
Identifieur interne : 002248 ( Main/Exploration ); précédent : 002247; suivant : 002249Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro.
Auteurs : Pengfei Wan [République populaire de Chine] ; Zunlai Sheng [République populaire de Chine] ; Qiang Han [République populaire de Chine] ; Yulin Zhao [République populaire de Chine] ; Guangdong Cheng [République populaire de Chine] ; Yanhua Li [République populaire de Chine]Source :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1873-376X ] ; 2014.
Descripteurs français
- KwdFr :
- Adsorption (MeSH), Antioxydants (composition chimique), Antioxydants (isolement et purification), Antioxydants (pharmacologie), Extraits de plantes (composition chimique), Extraits de plantes (isolement et purification), Extraits de plantes (pharmacologie), Flavonoïdes (composition chimique), Flavonoïdes (isolement et purification), Flavonoïdes (pharmacologie), Médicaments issus de plantes chinoises (composition chimique), Médicaments issus de plantes chinoises (isolement et purification), Médicaments issus de plantes chinoises (pharmacologie), Populus (composition chimique), Porosité (MeSH), Résines synthétiques (composition chimique).
- MESH :
- composition chimique : Antioxydants, Extraits de plantes, Flavonoïdes, Médicaments issus de plantes chinoises, Populus, Résines synthétiques.
- isolement et purification : Antioxydants, Extraits de plantes, Flavonoïdes, Médicaments issus de plantes chinoises.
- pharmacologie : Antioxydants, Extraits de plantes, Flavonoïdes, Médicaments issus de plantes chinoises.
- Adsorption, Porosité.
English descriptors
- KwdEn :
- Adsorption (MeSH), Antioxidants (chemistry), Antioxidants (isolation & purification), Antioxidants (pharmacology), Drugs, Chinese Herbal (chemistry), Drugs, Chinese Herbal (isolation & purification), Drugs, Chinese Herbal (pharmacology), Flavonoids (chemistry), Flavonoids (isolation & purification), Flavonoids (pharmacology), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Plant Extracts (pharmacology), Populus (chemistry), Porosity (MeSH), Resins, Synthetic (chemistry).
- MESH :
- chemical , chemistry : Antioxidants, Drugs, Chinese Herbal, Flavonoids, Plant Extracts, Resins, Synthetic.
- chemical , isolation & purification : Antioxidants, Drugs, Chinese Herbal, Flavonoids, Plant Extracts.
- chemical , pharmacology : Antioxidants, Drugs, Chinese Herbal, Flavonoids, Plant Extracts.
- chemistry : Populus.
- Adsorption, Porosity.
Abstract
Enrichment and purification of total flavonoids from Flos Populi extracts were studied using five macroporous resins. The static tests indicated that NKA-9 resin was appropriate and its adsorption data were well fitted to the Langmuir and Freundlich isotherms. To optimize the separation process, dynamic adsorption and desorption tests were carried out. The optimal adsorption parameters were initial concentrations in sample solution of 7.64mg/mL, pH of 5.0, sample loading amount of 2.3BV, flow rate of 2BV/h, temperature of 25°C. The optimal desorption parameters were deionized water and 20% ethanol each 5BV, then 60% ethanol of 10 BV, flow rate of 2BV/h. After one run treatment with NKA-9 resin, the content of total flavonoids in the product increased from 11.38% to 53.41%, and the recovery yield was 82.24%. The results showed that NKA-9 resin revealed a good ability to enrichment total flavonoids from Flos Populi, and the method can be referenced for the enrichment of total flavonoids from other materials. The antioxidant activities of the purified flavonoids were further evaluated in vitro. It showed that the DPPH radical scavenging increased from 59.46% to 82.63% at different concentrations (0.06-0.14mg/mL). At different concentrations (0.6-1.4mg/mL), the hydroxyl radical scavenging increased from 35.39% to 74.12%. Moreover, the reducing ability and total oxidant capacity appeared to be dose-dependent of flavonoids. It indicated that the purified flavonoids can be used as a source of potential antioxidant.
DOI: 10.1016/j.jchromb.2013.11.033
PubMed: 24321763
Affiliations:
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Electronic address: shengzunlai@neau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Han, Qiang" sort="Han, Qiang" uniqKey="Han Q" first="Qiang" last="Han">Qiang Han</name><affiliation wicri:level="1"><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Yulin" sort="Zhao, Yulin" uniqKey="Zhao Y" first="Yulin" last="Zhao">Yulin Zhao</name><affiliation wicri:level="1"><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Guangdong" sort="Cheng, Guangdong" uniqKey="Cheng G" first="Guangdong" last="Cheng">Guangdong Cheng</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Jiamusi University, Jiamusi, 148 Xuefu Road, Jiamusi 154007, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Jiamusi University, Jiamusi, 148 Xuefu Road, Jiamusi 154007</wicri:regionArea><wicri:noRegion>Jiamusi 154007</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yanhua" sort="Li, Yanhua" uniqKey="Li Y" first="Yanhua" last="Li">Yanhua Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: liyanhua@neau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030</wicri:regionArea><wicri:noRegion>Harbin 150030</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of chromatography. 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The static tests indicated that NKA-9 resin was appropriate and its adsorption data were well fitted to the Langmuir and Freundlich isotherms. To optimize the separation process, dynamic adsorption and desorption tests were carried out. The optimal adsorption parameters were initial concentrations in sample solution of 7.64mg/mL, pH of 5.0, sample loading amount of 2.3BV, flow rate of 2BV/h, temperature of 25°C. The optimal desorption parameters were deionized water and 20% ethanol each 5BV, then 60% ethanol of 10 BV, flow rate of 2BV/h. After one run treatment with NKA-9 resin, the content of total flavonoids in the product increased from 11.38% to 53.41%, and the recovery yield was 82.24%. The results showed that NKA-9 resin revealed a good ability to enrichment total flavonoids from Flos Populi, and the method can be referenced for the enrichment of total flavonoids from other materials. The antioxidant activities of the purified flavonoids were further evaluated in vitro. It showed that the DPPH radical scavenging increased from 59.46% to 82.63% at different concentrations (0.06-0.14mg/mL). At different concentrations (0.6-1.4mg/mL), the hydroxyl radical scavenging increased from 35.39% to 74.12%. Moreover, the reducing ability and total oxidant capacity appeared to be dose-dependent of flavonoids. It indicated that the purified flavonoids can be used as a source of potential antioxidant. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24321763</PMID><DateCompleted><Year>2014</Year><Month>09</Month><Day>03</Day></DateCompleted><DateRevised><Year>2014</Year><Month>01</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-376X</ISSN><JournalIssue CitedMedium="Internet"><Volume>945-946</Volume><PubDate><Year>2014</Year><Month>Jan</Month><Day>15</Day></PubDate></JournalIssue><Title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</Title><ISOAbbreviation>J Chromatogr B Analyt Technol Biomed Life Sci</ISOAbbreviation></Journal><ArticleTitle>Enrichment and purification of total flavonoids from Flos Populi extracts with macroporous resins and evaluation of antioxidant activities in vitro.</ArticleTitle><Pagination><MedlinePgn>68-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jchromb.2013.11.033</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1570-0232(13)00646-6</ELocationID><Abstract><AbstractText>Enrichment and purification of total flavonoids from Flos Populi extracts were studied using five macroporous resins. The static tests indicated that NKA-9 resin was appropriate and its adsorption data were well fitted to the Langmuir and Freundlich isotherms. To optimize the separation process, dynamic adsorption and desorption tests were carried out. The optimal adsorption parameters were initial concentrations in sample solution of 7.64mg/mL, pH of 5.0, sample loading amount of 2.3BV, flow rate of 2BV/h, temperature of 25°C. The optimal desorption parameters were deionized water and 20% ethanol each 5BV, then 60% ethanol of 10 BV, flow rate of 2BV/h. After one run treatment with NKA-9 resin, the content of total flavonoids in the product increased from 11.38% to 53.41%, and the recovery yield was 82.24%. The results showed that NKA-9 resin revealed a good ability to enrichment total flavonoids from Flos Populi, and the method can be referenced for the enrichment of total flavonoids from other materials. The antioxidant activities of the purified flavonoids were further evaluated in vitro. It showed that the DPPH radical scavenging increased from 59.46% to 82.63% at different concentrations (0.06-0.14mg/mL). At different concentrations (0.6-1.4mg/mL), the hydroxyl radical scavenging increased from 35.39% to 74.12%. Moreover, the reducing ability and total oxidant capacity appeared to be dose-dependent of flavonoids. It indicated that the purified flavonoids can be used as a source of potential antioxidant. </AbstractText><CopyrightInformation>Copyright © 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wan</LastName><ForeName>Pengfei</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sheng</LastName><ForeName>Zunlai</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: shengzunlai@neau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Yulin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Guangdong</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>College of Life Science, Jiamusi University, Jiamusi, 148 Xuefu Road, Jiamusi 154007, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yanhua</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: liyanhua@neau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>11</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Chromatogr B Analyt Technol Biomed Life Sci</MedlineTA><NlmUniqueID>101139554</NlmUniqueID><ISSNLinking>1570-0232</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000975">Antioxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004365">Drugs, Chinese Herbal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005419">Flavonoids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012117">Resins, Synthetic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000327" MajorTopicYN="N">Adsorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000975" MajorTopicYN="N">Antioxidants</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004365" MajorTopicYN="N">Drugs, Chinese Herbal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005419" MajorTopicYN="N">Flavonoids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016062" MajorTopicYN="N">Porosity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012117" MajorTopicYN="N">Resins, Synthetic</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antioxidant activity</Keyword><Keyword MajorTopicYN="N">Enrichment</Keyword><Keyword MajorTopicYN="N">Flos Populi</Keyword><Keyword MajorTopicYN="N">Macroporous resins</Keyword><Keyword MajorTopicYN="N">Total Flavonoids</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>07</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>11</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>11</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>12</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>12</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24321763</ArticleId><ArticleId IdType="pii">S1570-0232(13)00646-6</ArticleId><ArticleId IdType="doi">10.1016/j.jchromb.2013.11.033</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wan, Pengfei" sort="Wan, Pengfei" uniqKey="Wan P" first="Pengfei" last="Wan">Pengfei Wan</name></noRegion><name sortKey="Cheng, Guangdong" sort="Cheng, Guangdong" uniqKey="Cheng G" first="Guangdong" last="Cheng">Guangdong Cheng</name><name sortKey="Han, Qiang" sort="Han, Qiang" uniqKey="Han Q" first="Qiang" last="Han">Qiang Han</name><name sortKey="Li, Yanhua" sort="Li, Yanhua" uniqKey="Li Y" first="Yanhua" last="Li">Yanhua Li</name><name sortKey="Sheng, Zunlai" sort="Sheng, Zunlai" uniqKey="Sheng Z" first="Zunlai" last="Sheng">Zunlai Sheng</name><name sortKey="Zhao, Yulin" sort="Zhao, Yulin" uniqKey="Zhao Y" first="Yulin" last="Zhao">Yulin Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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